News Updates

Diffinity Genomics acquired by Chiral Technologies. 

Diffinity Genomics issued patent for their technology "Spatially inhomogenously functionalized porous media and method for use in selective removal of contaminants".  The patent was issued June 23, 2015. 

Technical Publications

Diffinity Genomics has developed the fastest PCR cleanup product on the market today.      

Diffinity Genomics’ patented technology is a novel method designed to selectively remove dNTPs,              primers and primer-dimers from PCR-amplified fragments, while providing up to 90% recovery of high-quality DNA. This methodology allows simple, rapid, and inexpensive cleanup inside a pipette tip.  

RapidTips® are functionalized pipette tips that contain Diffinity resin. Using a simple, one-step protocol, Diffinity RapidTips cleanup PCR fragments in just one minute.

There are many technical publications that describe use of RapidTips for a variety of genomics applications.

Read our newest publications about sequencing of chicken genome; you can also read about a new approach to genome editing published in Nature by a Harvard University team.  

Quantification of allele specific expression (ASE) for two alleles of the HTR2B gene in different broiler chickens and different tissues

Validation of a exonuclease genotyping for a pair of SNPs within the chicken FNDC9 gene

Programmable editing of a target base in genomic DNA without a double-stranded DNA cleavage, Alexis C. Komor, Yongjoo B. Kim, Michael S. Packer, John A. Zuris & David R. Liu, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, Nature volume533pages420424 (19 May 2016) doi:10.1038/nature17946


International Space Station environmental microbiome — microbial inventories of ISS filter debris, Kasthuri Venkateswaran, Parag Vaishampayan, Jessica Cisneros, Duane L. Pierson, Scott O. Rogers, Jay Perry;  Applied Microbiology and Biotechnology; July 2014Volume 98, Issue 14, pp 6453-6466Date: 04 Apr 2014

Frequent Replenishment Sustains the Beneficial Microbiome of Drosophila melanogaster, Blum JE, Fischer CN, Miles J, Handelsman J.; MBio. 2013 Nov 5;4(6). pii: e00860-13. doi: 10.1128/mBio.00860-13.

Nitrogen deposition alters plant-fungal relationships: linking belowground dynamics to aboveground vegetation change, Dean, S. L., Farrer, E. C., Taylor, D. L., Porras-Alfaro, A., Suding, K. N. and Sinsabaugh, R. L. (2014), Nitrogen deposition alters plant–fungal relationships: linking belowground dynamics to aboveground vegetation change. Mol Ecol, 23: 1364–1378. doi:10.1111/mec.12541

Factors influencing Dipylidium sp. infection in a free-ranging social carnivore, the spotted hyaena (Crocuta crocuta), Marion L. East, Christoph Kurze, Kerstin Wilhelm, Sarah Benhaiem, Heribert Hofer, International Journal for Parasitology: Parasites and Wildlife, Available online 18 September 2013, ISSN 2213-2244,

Operational aspects, pH transition and microbial shifts of a H2S desulfurizing biotrickling filter with random packing material, Andrea M. Montebello, Tercia Bezerra, Roger Rovira, Laura Rago, Javier Lafuente, Xavier Gamisans, Susana Campoy, Mireia Baeza, David Gabriel, Chemosphere, Available online 13 September 2013, ISSN 0045-6535,

Effects of a dietary β-(1,3)(1,6)-D-glucan supplementation on intestinal microbial communities and intestinal ultrastructure of mirror carp, (Cyprinus carpio L.) Kühlwein, H., Emery, M.J., Rawling, M.D., Harper, G.M., Merrifield, D.L. and Davies, S.J. (2013), Journal of Applied Microbiology. doi: 10.1111/jam.12313

The biogeography of the yeti crabs (Kiwaidae) with notes on the phylogeny of the Chirostyloidea (Decapoda: Anomura), C.N. Roterman, J.T.Copley, K.T Linse, P.A.Tyler, and A.D.Rogers  Proc R Soc B 2013 280: 20130718

A Cell Line Resource Derived from Honey Bee (Apis mellifera) Embryonic TissuesGoblirschMJ, SpivakMS, KurttiTJ (2013). PLoSONE 8(7):e69831. doi:10.1371/journal.pone.0069831

Genetic characterization of small ruminant lentiviruses circulating in naturally infected sheep and goats in Ontario, CanadaLisa A. Santry, Jondavid de Jong, Alexander C. Gold, Scott R. Walsh,  Paula I. Menzies, Sarah K. Wootton; Virus Research (2013), Available online 10 April 2013, In Press, Corrected Proof; '

New perspectives on viable microbial communities in low-biomass cleanroom environments, Parag Vaishampayan, Alexander J Probst, Myron T La Duc, Emilee Bargoma, James N Benardini, Gary L Andersen and Kasthuri Venkateswaran,The ISME Journal (2013) 7, 312–324; doi:10.1038/ismej.2012.114; published online 11 October 2012

The microbiome extends to subepidermal compartments of normal skin, Teruaki Nakatsuji, Hsin-I. Chiang, Shangi B. Jiang, Harish Nagarajan, Karsten Zengler & Richard L. Gallo; Journal name: Nature Communications Volume: 4, Article number: 1431 DOI: doi:10.1038/ncomms2441 Received 28 August 2012 ; Accepted 19 December 2012  Published 05 February 2013

Pyrosequencing-Derived Bacterial, Archaeal, and Fungal Diversity of Spacecraft Hardware Destined for Mars, Myron T. La Duc, Parag Vaishampayan, Henrik R. Nilsson, Tamas Torok, and Kasthuri Venkateswaran; Appl. Environ. Microbiol. August 2012 78:5912-5922; published ahead of print 22 June 2012, doi:10.1128/AEM.01435-12

Biosynthesis and characterization of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from Bacillus cereus FA11 isolated from TNT-contaminated soilFarha Masood, Fariha Hasan, Safia Ahmed, Abdul Hameed; Annals of Microbiology DOI 10.1007/s13213-011-0386-3; Received: 13 June 2011 / Accepted: 9 November 2011# Springer-Verlag and the University of Milan 2011

Phylomark, a Tool To Identify Conserved Phylogenetic Markers from Whole-Genome Alignments, Jason W. Sahl, Malcolm N. Matalka, and David A. Rasko, Appl. Environ. Microbiol. July 2012 vol. 78 no. 14 4884-4892; Published ahead of print 11 May 2012, doi: 10.1128/​AEM.00929-12

New perspectives on viable microbial communities in low-biomass cleanroom environments, Parag Vaishampayan1,4, Alexander J Probst2,4, Myron T La Duc1,4, Emilee Bargoma1,James N Benardini1, Gary L Andersen3 and Kasthuri Venkateswaran11Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology,Pasadena, CA, USA; 2Department for Microbiology and Archaea Center, University of Regensburg,Regensburg, Germany and 3Earth Sciences Division, Ecology Department, Lawrence Berkeley National Laboratory, Berkeley, CA, USAThe ISME Journal (2013) 7, 312–324& 2013 International Society for Microbial Ecology All rights reserved 1751-7362/13

Comparison of Culture and Molecular Identification of Bacteria in Chronic Wounds, Daniel D. Rhoads 1,2, Randall D. Wolcott 1,3,4,*, Yan Sun 3 and Scot E. Dowd 3,4 1 Southwest Regional Wound Care Center, Lubbock, TX 79410, USA2 Texas Tech University Health Sciences Center School of Medicine, Lubbock, TX 79415, USA3 Research and Testing Laboratory, Lubbock, TX 79407, USA4 Pathogenius Laboratory, Lubbock, TX 79407, USA  *Author to whom correspondence should be addressed; Tel.: +1-806-793-8869. Received: 1 November 2011; in revised form: 30 January 2012 / Accepted: 13 February 2012 / Published: 23 February 2012 Int. J. Mol. Sci. 2012, 13(3), 2535-2550; doi:10.3390/ijms13032535

Granulomas Caused by Mycobacterium sp. in farmed turbot Scopthalmus maximus (Linnaeus, 1758), D. JERONIMO1, A. BARBOSA1, M. RAMOS1,2, J. MARQUES1,2, M.H. MOREIRA1, J.A. SOUSA1,2, C. CRUZ1,2 and A. SARAIVA1,2 1 Faculdade de Ciências, Universidade do Porto, Departamento de Biologia, Rua do Campo Alegre, Edifício FC4, 4169-007 Porto, Portugal 2 CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. Corresponding author: Received: 6 August 2012; Accepted: 29 August 2013; Published on line: 25 September 2013. Mediterranean Marine Science Indexed in WoS (Web of Science, ISI Thomson) and SCOPUS. The journal is available on line at DOI:

Scopulariopsis, a Poorly Known Opportunistic Fungus: Spectrum ofSpecies in Clinical Samples and In Vitro Responses to Antifungal Drug, Marcelo Sandoval-Denis,a Deanna A. Sutton,b Annette W. Fothergill,b Josep Cano-Lira,a Josepa Gené,a C. A. Decock,c G. S. de Hoog,dJosep GuarroaUnitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spaina; Fungus Testing Laboratory, University of Texas HealthScience Center, San Antonio, Texas, USAb; Mycothèque de l’Université Catholique de Louvain (MUCL, BCCMTM), Louvain-la-Neuve, Belgiumc; CBS-KNAW Fungal; Biodiversity Centre, Utrecht, The Netherlands December 2013 Volume 51 Number 12

METAGENOMICS AND METATRANSCRIPTOMICS OF A H2S DESULFURIZING BIOTRICKLING FILTER, TERCIA BEZERRA*, ROGER ROVIRA*, ANDREA MONTEBELLO* MONTSERRAT LLAGOSTERA**, JAVIER LAFUENTE*, SUSANA CAMPOY**, DAVID GABRIEL* *Department of Chemical Engineering, Universitat Autònoma de Barcelona, Barcelona, Spain **Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Barcelona, Spain Corresponding author:, +34935811587

Lack of Vitamin D Receptor Causes Dysbiosis and Changes the Functions of the Murine Intestinal Microbiome, Dapeng Jin, MS1, Shaoping Wu, PhD1, Yong-guo Zhang, PhD1, Rong Lu, PhD1, Yinglin Xia, PhD2,Hui Dong, PhD3, Jun Sun, PhD1, , 1 Department of Biochemistry, Rush University Medical Center, Chicago, Illinois2 Department of Biostatistics and Computational Biology, University of Rochester, Rochester, New York3 Department of Gastroenterology, Xingqiao Hospital, The Third Military Medical University, Chongqing, China. Accepted 9 April 2015, Available online 2 June 2015; Clinical Therapeutics, Volume 37, Issue 5, 1 May 2015, Pages 996–1009.e7

Genetic analysis of amyotrophic lateral sclerosis in the Slovenian population, Katarina Vrabeca, Blaž Koritnikb,Lea Leonardisb,Leja Dolenc-Grošeljb,Janez Zidarb,Bradley Smithc,Caroline Vancec,Christopher Shawc,Boris Rogeljd, e,Damjan Glavača,Metka Ravnik-Glavača, Corresponding author at: Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia. Tel.: +386 1 543 7183; fax: +386 1 543 7181. Neurobiology of Aging Volume 36, Issue 3, March 2015, Pages 1601.e17–1601.e20