Diffinity Genomics Sequence Detection Kit
The Diffinity genomics genotyping kit is a fast, sensitive system to detect sequence specific amplification in PCR reactions.
- Replaces electrophoresis for routine PCR genotyping
- Simplifies SNP assays without real time PCR
Two Step Procedure Yields Colorimetric or Fluorometric Detection of Target Sequences in 5 Minutes
- Step 1: Add an unlabeled oligonucleotide probe of your own design to your PCR amplified sample. Incubate 2 minutes at room temperature.
- Step 2: Add Diffinity’s patent-pending reagents to bind unhybridized probes.
- Read the Results.
Detection of DNA, RNA, and SNPs for a Variety of Applications
- Any target sequence capable of binding a unique DNA oligo probe can be detected with the kit.
- Application to RNA structure determination, SNP detection, routine genotyping, gene expression.
The Diffinity Genomics Sequence Detection Kit is an accurate, sensitive replacement for electrophoresis when information about PCR amplicon size is not required. The kit allows the user to determine the nucleotide sequence under test, with the fluorescence test requiring that the oligo probe contain a fluorophor. The colorimetric assay produces a color change observable without instrumentation with as little as 100 fmols (10-13 moles) of target sequence in a reaction mixture1. The fluorometric assay is much more sensitive, allowing the use of multiple fluorescently labeled probes in a single sample2.
The patent-pending technology of the Sequence Detection Kit takes advantage of the property of colloidal solutions of gold nanoparticles to preferentially and irreversibly bind single stranded DNA in aqueous solution. Importantly, this electrostatic binding efficiently quenches fluorophores associated with these oligo probes. This binding reaction happens at room temperature and does not require instrumentation or long incubation times.
Diffinity Genomics, Inc. West Henrietta, NY
(585) 214-0585
References:
- Li, H., and Rothberg, L. (2004) Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc Natl Acad Sci U S A. 101(39):14036-9.
- Li, H., and Rothberg, L.J. (2004) DNA sequence detection using selective fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles. Anal Chem 76(18):5414-7.
- Li H, Rothberg LJ. (2004) Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction. J Am Chem Soc. 2004 Sep 8;126(35):10958-61.
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