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Diffinity Genomics acquired by Chiral Technologies.  Read more...

Diffinity Genomics issued patent for their technology "Spatially inhomogenously functionalized porous media and method for use in selective removal of contaminants".  The patent was issued June 23, 2015. 
 

Diffinity DNA RapidExtract Kit - FAQs

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Diffinity DNA RapidExtract™ Kit - Frequently Asked Questions

Q. How long can you store the extracted samples at -20° C? 
A. The extracted samples are stable for at least one month at -20° C. 

Q. Can DNA extracted using the Diffinity DNA RapidExtractTM Kit be used as template in real-time PCR?
A.    The DNA extracted using the Diffinity DNA RapidExtractTM Kit can be used in real-time PCR. However, residual inhibitors may quench fluorophore dyes used in real-time PCR analysis.

Q. Can DNA extracted using the Diffinity DNA RapidExtractTM Kit be used as template in DNA amplification reactions other than PCR?
A. In addition to PCR, DNA extracted using Diffinity DNA RapidExtractTM Kit can be amplified in isothermal DNA amplification reactions including LAMP and ICAN.

Q. Can DNA polymerases other than “Kaneka High-Speed DNA Polymerase” be used in PCR reactions with DNA extracted using the Diffinity DNA RapidExtractTM Kit as template?
A.    Other manufacturers’ DNA polymerases will work with DNA extracted using the Diffinity DNA RapidExtractTM” Kit, but may exhibit lower PCR efficiency. For best performance, the use of polymerases with high tolerance to impurities is recommended.  

Q. Is incubation at 98 required for the sample lysis step?
A. Lysis performed at temperatures lower than 98 may decrease DNA extraction efficiency.

Q. Can the Diffinity DNA RapidExtractTM Kit also be used for RNA extraction?
A. The Diffinity DNA RapidExtractTM Kit is not optimized for RNA extraction. 

Q. What are the storage recommendations for the Diffinity DNA RapidExtractTM Kit? Is it possible to store the Diffinity DNA RapidExtractTM Kit in a refrigerator?
A. The Diffinity DNA RapidExtractTM Kit should be stored at room temperature away from direct sunlight.

Q. Is it possible to determine the amount of DNA in the extract solution by spectrophotometry?
A. Impurities derived from samples may affect the absorption measurement. When absolutely necessary, it is possible to improve the accuracy of measurements by the protocol described below:

1.    Centrifuge the extract at 5000 rpm for 5 minutes.

2.    Dilute the supernatant 2- to 10-fold with distilled water.

3.    Measure the absorption of diluted supernatant.

Q. How can low PCR-amplification efficiency be improved?
A. If PCR amplification is not evident or exhibits low efficiency, check our new DNA RapidExtract kit Troubleshooting Guide.