DNA Purification

The first application of Diffinity Genomics' nucleic acid purification technology is DNA purification. DNA purification techniques are commonly used on PCR fragments, plasmids, and genomic DNA to prepare samples for downstream reactions. For example; after PCR cleanup, DNA can be used for sequencing, cloning by restriction digest, in vitro transcription, or many other applications. Circular plasmid DNA is used frequently in research to grow specific sequences in bacteria and subsequently express those genes in tissue culture. Genomic DNA is used to generate PCR and plasmid DNA samples as well as for forensics and mutation analysis.

Our first product, the Diffinity RapidTip, will remove nucleotides, primers, and primer dimers to purify PCR solution prior to Sanger sequencing.  RapidTip prototypes have been enthusiastically received by researchers at renowned genomics centers.

Future DNA purification applications of Diffinity’s technology could include the following: