Diffinity Genomics acquired by Chiral Technologies. Read more...
Diffinity Genomics issued patent for their technology "Spatially inhomogenously functionalized porous media and method for use in selective removal of contaminants". The patent was issued June 23, 2015.
Diffinity RapidTip and RapidTip2: The Future of PCR Cleanup!
The data below shows why YOU should consider RapidTips for your PCR cleanup needs.
Diffinity RapidTip2 effectively removes dNTPs (not visible with gel), primers (20bp), and primer-dimers (<40bp). The PCR product was divided and subjected to PCR cleanup with either competitors products (C) or RapidTip2. The RapidTip2 has high yield: the PCR amplicon (2kb) shows no loss of band after cleanup.
Diffinity RapidTip2 effectively removes DNA polymerase comparably to popular spin column methods. PCR products were prepared and cleaned via a competitor’s product (C), RapidTip2 (R), or left uncleaned (U). Then the products were placed in a thermocycler. Our sensitive assay generates a 600bp band that indicated polymerase is present. The control and RapidTip2 lanes have no detectable 600bp indicating that polymerase was adequately removed.
The Diffinity RapidTip2 has excellent DNA yield (up to 90%). This diagram shows equal volumes of PCR product. The uncleaned sample is represented by (-) and the Diffinity RapidTip treated sample is represented by (+). The treated sample shows no loss of band intensity. They were run in an agarose gel alongside Invitrogen 1Kb Plus Ladder (L) and labeled with SYBER Safe.
PCR cleanup using Diffinity RapidTip is Fast!
96 samples were cleaned in batches of 8 using an 8-channel pipette and following each manufacturer’s protocol. For even faster cleanup, use a 96-head pipettor or benchtop pipetting system to clean a plate of PCR samples in less than 2 minutes!
RapidTip cleanup enables excellent Sanger sequencing. Read lengths are used to quantify sequence quality and compare clmethods. Quality value (QV20) is the number of bases with an error rate less than 1% while the continuous read length (CRL) is the number of adjacent bases with an error rate of less than 1%.
The Diffinity RapidTip technology improves sequence quality as good as or better than the competition, in one minute using just a functionalized pipette tip. It improves peak quality with low background.
All you need for PCR cleanup - RapidTips and a pipettor!
It's all in the tip! Everything you need for PCR cleanup is contained within our functionalized RapidTip or RapidTip2. No additional reagents, consumables or equipment are needed. All you need is one of Diffinity's Rapidtips and a pipettor for a fast, easy, cost-effective PCR cleanup method!
Everything you need for
spin column cleaning
Reagents, centrifuge, multiple consumables are needed, and along with multiple protocol steps. The spin column cleaning method takes longer and produces more waste than RapidTip cleaned samples.
Chiral Technologies, Inc. Diffinity Genomics ®
800 North Five Points Road
West Chester, PA 19380