|
Diffinity RapidTip and RapidTip2 represent the Future of PCR Purification. The data below shows why YOU should consider RapidTips for your PCR purification needs!
|
Diffinity RapidTip2 effectively removes dNTPs (not visible with gel), primers (20bp), and primer-dimers (50bp). The PCR product was divided and subjected to PCR purification with either competitors products (C) or RapidTip2. The RapidTip2 has high yield: the PCR amplicon (2kb) shows no loss of band after purification.
|
 |
|
Diffinity RapidTip2 effectively removes DNA polymerase. Diffinity RapidTip2 purifies PCR solutions and removes DNA polymerase (standard, Hot Start, and Proofreading) comparably to popular spin column methods. PCR reactions were prepared and purified via a competitor’s product (C), RapidTip2 (R), or left unpurified (U). Then the reactions were placed in a thermocycler. Our sensitive assay generates a 600bp band that indicated polymerase is present. The control and RapidTip2 lanes have no detectable 600bp indicating that polymerase was adequately removed.
|
 |
|
The Diffinity has excellent DNA yield (up to 90%). This diagram shows equal volumes of PCR product. The unpurified sample is represented by (-) and the Diffinity RapidTip treated sample is represented by (+). They were run in an agarose gel alongside Invitrogen 1Kb Plus Ladder (L) and labeled with SybrSafe.
|
 |
|
Diffinity RapidTip purification is Fast!
96 samples were purified in batches of 8 using an 8-channel pipette and following each manufacturer’s protocol. For even faster purification, use a 96-head pipettor, benchtop pipetting system, or automated liquid handling robot to purify a plate of PCR samples in less than 2 minutes.
|
 |
| RapidTip purification enables excellent Sanger sequencing. Read lengths are used to quantify sequence quality and compare purification methods. Qualit y value (QV20) is the number of bases with an error rate less than 1% while the continuous read length (CRL) is the number of adjacent bases with an error rate of less than 1% |
 |
|
The Diffinity RapidTip technology improves sequence quality as good as or better than the competition, in one minute just using a functional pipette tip. It improves peak quality with low background.
|
 |
|
All you need for RapidTip Purification + a pipettor!
It's all in the tip! Everything you need for PCR Purification is contained within our functional RapidTip or RapidTip2. No additional reagents, consumables or equipment are needed for purificaiton. All you need is one of Diffinity's functional tips and a pipettor for a fast, easy, cost-effective PCR Purification method!
|
Everything you need for Spin Column purification
|
|
